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Description
Immunofluorescence Mice brain sections were boiled in citric acid buffer (pH = 6.0) for 10 min for antigen repair
The agarose gel was washed for 5 min in distilled water, stained for 30 min with 0.5 g/mL ethidium bromide, and destained for 5 min in distilled water before imaging
Consequent alterations in the immune response create a microenvironment that facilitates a climax chronic community having a reduced growth rate and being inherently resistant to antibiotic therapy (56, 57)
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