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Description
acnes-induced inflammatory reactions in vitro and in vivo
Key detail: B12 deficiency symptoms overlap completely with diabetic neuropathy, so always check B12 levels before accepting a neuropathy diagnosis
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Primary antibodies were incubated in diluted (1%) blocking buffer overnight at dilutions 1/100 or 1/200 at 4 C, washed with PBS-T and incubated with secondary antibodies diluted 1/200 or 1/500 in 1% diluted buffer for one hour at room temperature, subsequently washed with PBS-T, counterstained with DAPI 1 ug/mL in PBS for five minutes, washed with PBS, and mounted using mounting media, and imaged the same day or the day after using Zeiss LSM-710 confocal microscope
AOD 9604 is a synthetic 16-amino-acid fragment of human growth hormone (residues 176191), developed for studying fat and energy metabolism
