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SCFAs supplementation has been demonstrated to reduce the levels of NLRP3 and IL-1, promote the IRF4/STAT3 complex and attenuate systemic inflammatory responses (Su et al., 2022), which may improve HFpEF

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The % Cytotoxicity was calculated using the following formula: L-carnitine oral challenge Seven weeks before the start of the study, mice were housed in groups of four according to sex, APOE genotype, and treatment group (Supplementary Table S3)

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The unexpected finding was that the contractile response was both smaller in amplitude and area under the curve in the presence of TMAO (Figures 9B, C), which contrasted with the Ca 2+ current (Figures 6, 7) and the Ca 2+ transients (Figure 8)