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Ethics declarations Ethics approval and consent to participate The Animal Welfare Committee of the Oswaldo Cruz Foundation (CEUA-FIOCRUZ) approved the experimental protocol under license number L-012/2021

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The chronic subcutaneous administration of Ex-4 attenuates the peripheral symptoms of type 2 diabetes in rats, including hyperglycemia and IR, and enhances the levels of brain cortical GLP-1 and insulin-like growth factor (IGF)-1 and their downstream signaling pathways

Key Findings: Participants: 338 obese adults Duration: 48 weeks Average weight loss: 17.5% to 24.2% depending on dose Metabolic improvements: Better insulin sensitivity, reduced inflammation markers These results put Retatrutide ahead of many current obesity drugs, making it one of the most promising new weight loss peptides of 2025

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